Systems and methods for preparing a polypeptide from a mixture
AssigneeRegeneron Pharmaceuticals, Inc.
InventorsIsabelle Livigni, Stefanie McDermott, James Reilly, John Mattila
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Abstract
Embodiments of the present disclosure are directed to methods for preparing a target polypeptide from a mixture including the target polypeptide. The method may include contacting the mixture to a hydrophobic interaction chromatography (HIC) apparatus including multiple chromatographic zones. The method may further include passing the target polypeptide through the outlets of at least a first zone and a second zone of the HIC apparatus. A residence time for the mixture including the target polypeptide in a first zone may be approximately the same as a residence time of one or more mobile phases in the second zone.
Claims11
A method for preparing a target polypeptide from a mixture including the target polypeptide, the method comprising:
- passing the mixture including the target polypeptide to a first column of a plurality of chromatographic columns in a hydrophobic interaction chromatography (HIC) apparatus, wherein each of the plurality of columns comprises an outlet connectable to another column of the plurality of columns;
- passing an effluent including the target polypeptide from the first column to a second column of the plurality of columns;
- passing one or more mobile phases to a third column of the plurality of columns;
- and
- passing the target polypeptide through the outlets of each of the plurality of columns, wherein a sum of residence times for the mixture including the target polypeptide in the first column and second column is approximately the same as the sum of the residence times of the one or more mobile phases in the third column.
Dependencies are inferred from the claim text.