Methods for purification of messenger RNA
AssigneeTranslate Bio, Inc.
InventorsFrank DeRosa, Anusha Dias, Michael Heartlein, Shrirang Karve
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Abstract
The present invention provides, among other things, methods of purifying messenger RNA (mRNA) including the steps of (a) precipitating mRNA from an impure preparation; (b) subjecting the impure preparation comprising precipitated mRNA to a purification process involving membrane filtration such that the precipitated mRNA is captured by a membrane; and (c) eluting the captured precipitated mRNA from the membrane by re-solubilizing the mRNA, thereby resulting in a purified mRNA solution. In some embodiments, a purification process involving membrane filtration suitable for the present invention is tangential flow filtration.
Claims16
A method of purifying messenger RNA (mRNA), comprising
- (a) precipitating mRNA;
- (b) treating the precipitated mRNA to a membrane filtration process;
- and
- (c) eluting the precipitated mRNA from the membrane by re-solubilizing the mRNA;
- wherein the membrane filtration process is tangential flow filtration, and wherein the method further comprises a step of dialyzing the purified mRNA solution.
Dependencies are inferred from the claim text.