Built-in bio-sleeve and preparation method and use thereof

A preparation method of an acellular allogeneic dermal matrix, characterized in that, the method comprises the following steps:

• Step 1), an allogeneic skin material is put into a solution of an enzyme(s), soaked and subjected to shaking treatment to obtain a semi-finished product A, wherein the enzyme is phospholipase, or the enzymes are phospholipase and protease;
• Step 2), the semi-finished product A is taken out, soaked with physiological saline, and subjected to shaking treatment to obtain a semi-finished product B;
• Step 3), the semi-finished product B is put into a surfactant solution, and subjected to ultrasonic soaking treatment to obtain a semi-finished product C;
• Step 4), the semi-finished product C is soaked with physiological saline, and subjected to shaking treatment to obtain a semi-finished product D;
• Step 5), the semi-finished product D is put into a container containing a DNA hydrolase solution, and subjected to shaking treatment to obtain a semi-finished product E;
• Step 6), the semi-finished product E is soaked with physiological saline, and subjected to shaking treatment to obtain a semi-finished product F;
• and
• Step 7):
• the semi-finished product F is washed and soaked with water for injection to obtain a finished product G.