Method for culturing 3-dimensional lung cancer organoid and method for preparing patient-derived xenograft animal model using same

A method for preparing a patient-derived lung cancer organoid xenograft animal model, comprising the steps of:

• (a) culturing lung cancer cells, which have been obtained by cellizing lung cancer tissue isolated from a lung cancer patient, with a cell culture substrate, to obtain a lung cancer organoid;
• (b) subjecting the obtained organoid to treatment with an enzyme and then allowing reaction to proceed;
• (c) adding, to the lung cancer organoid after step (b), a cell culture substrate, and performing culture so that a 3-dimensional lung cancer organoid is formed;
• (d) removing the cell culture substrate from the 3-dimensional lung cancer organoid;
• (e) encapsulating the 3-dimensional lung cancer organoid, from which the cell culture substrate has been removed, in a membrane and then solidifying the same;
• and
• (f) transplanting, into a subject animal, the 3-dimensional lung cancer organoid obtained in step (e), wherein the transplanting into a subject animal does not comprise injection of the 3-dimensional lung cancer organoid by syringe.