Methods of eliciting antibodies that bind to full-length glycosylated HIV-1 Env using multimerized Env cores
AssigneeFred Hutchinson Cancer Center
InventorsLeonidas Stamatatos, Andrew McGuire, Katherine Rachael Parks, Matthew D. Gray
Life of this patent
Estimating the term…
Abstract
Sequential immunization strategies to guide the maturation of antibodies against the human immunodeficiency virus (HIV) are described. The sequential immunization strategies utilize an HIV envelope protein (Env) that binds germline (gl) B cells as a first (prime) immunization and an Env with a functional glycosylated N276 as a second (boost) immunization. The sequential immunization strategies successfully elicit neutralizing antibodies against HIV.
Claims13
A method of eliciting antibodies that bind full length glycosylated human immunodeficiency virus (HIV)-1 envelope protein (Env) comprising administering to a subject:
- (i) a prime immunization comprising a multimerized 426c core HIV-1 Env;
- and
- (ii) a boost immunization comprising a multimerized HxB2 core HIV-1 Env with a glycosylated N276 wherein no Env are administered to the subject between the (i) prime immunization and (ii) boost immunization
- thereby eliciting antibodies that bind full length glycosylated HIV-1 Env
- wherein residue numbering is based on the prototypic HIV-1 isolate HxB2.
Dependencies are inferred from the claim text.