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US 11,883,481 B2Granted
Recombinant herpesvirus of turkey vectors expressing antigens of avian pathogens and uses thereof
AssigneeZoetis Services LLC
InventorsSing Rong, Yugang Luo, Tyler Brown
Life of this patent
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Abstract
The invention relates to recombinant viral vectors for the insertion and expression of foreign genes for use in safe immunizations to protect against a variety of pathogens. The invention also relates to multivalent compositions or vaccine comprising one or more recombinant viral vectors for protection against a variety of pathogens. The present invention relates to methods of making an using said recombinant viral vectors.
Claims24
A recombinant Herpesvirus of Turkey (HVT) genome comprising:
- a. a nucleotide sequence coding for a heterologous antigen from an Infectious Bursal Disease Virus inserted into the intergenic loci UL 35/UL 36 in the unique long region of the HVT genome:
- and
- b. a nucleotide sequence coding for a heterologous antigen from a Newcastle Disease Virus inserted at the UL55/Gene 3 site in the unique long region (UL) of the HVT genome.
A recombinant Herpesvirus of Turkey (HVT) genome comprising:
- a. a nucleotide sequence coding for an Infectious Bursal Disease Virus VP2 protein comprising at least 80% sequence identity to the nucleotide sequence that comprises either SEQ ID NO.5 or SEQ ID NO. 10 inserted into the intergenic loci UL 35/UL 36 in the unique long region of the HVT genome;
- and
- b. a nucleotide sequence coding for the Newcastle Disease Virus F protein comprising at least 80% sequence identity to the nucleotide sequence that comprises SEQ ID NO. 3 inserted at the UL55/Gene 3 site in the unique long region (UL) of the HVT genome.
A recombinant Herpesvirus of Turkey (HVT) genome comprising:
- a. an expression cassette inserted into the intergenic loci UL 35/UL 36 of the unique long region of the HVT genome comprising, in order:
- a nucleotide sequence for the human cytomegalovirus promoter comprising SEQ ID NO. 1, the nucleotide sequence coding for the Infectious Bursal Disease Virus VP2 protein comprising a nucleotide sequence comprising at least 80% sequence identity to the nucleotide sequences comprising either SEQ ID NO.5 or SEQ ID NO. 10, and the nucleotide sequence coding for the Bovine Growth Hormone (BGH) poly A tail comprising SEQ ID NO.6;
- and
- b. an expression cassette inserted into the UL55/Gene 3 site of the unique long region of the HVT genome comprising, in order:
- the nucleotide sequence for the murine cytomegalovirus promoter comprising SEQ ID NO. 2;
- the nucleotide sequence coding for the Newcastle Disease Virus F protein comprising a nucleotide sequence comprising at least 80% sequence identity to the nucleotide sequence comprising SEQ ID NO. 3, and the nucleotide sequence coding for the SV40 poly A tail comprising SEQ ID NO. 12.
3 more elements
Dependencies are inferred from the claim text.