Microbiome protection from oral antibiotics

A method of protecting a subject's gastrointestinal (GI) microbiome from an oral antibiotic, comprising administering a formulation comprising a beta-lactamase capable of deactivating the oral antibiotic to a subject who is undergoing treatment or has recently undergone treatment with the oral antibiotic, wherein:

• the formulation comprises an enterically coated capsule comprising an enterically coated pellet, the pellet comprising, relative to a pellet's weight:
• about 20% to 25% sucrose sphere;
• about 30% to 40% binder excipient;
• about 12% to 18% beta-lactamase;
• about 1% to 2% buffer salts;
• about 0.5% to 3% plasticizer;
• and
• about 15% to 30% EUDRAGIT® (poly(methacrylic acid, methylmethacrylate) coating,
• wherein the oral antibiotic is co-formulated with one or more beta-lactamase inhibitors.