Xenograft soft tissue implants and methods of making

A surgical method of using a graft comprising:

• implanting the graft during surgery, for treatment of a human patient;
• wherein the graft is prepared by:
• (a) providing a bovine dermis having a native dermal matrix;
• (b) treating the bovine dermis with an aqueous alkaline sodium sulfide solution for at least 1 hour, wherein the aqueous alkaline sodium sulfide solution comprises 0.01% to 1.5% by weight of sodium sulfide and 0.001 M to 0.5 M sodium hydroxide, wherein a pH of the aqueous alkaline sodium sulfide solution is greater than 10;
• (c) treating the bovine dermis with a saline solution at least once;
• (d) treating the bovine dermis with an aqueous hydrogen peroxide solution at least once;
• (e) dehydrating the bovine dermis via solvent dehydration with a solvent starting with a first concentration of the solvent and finishing with a second concentration of the solvent, higher than the first concentration of the solvent;
• and
• (f) maintaining a histoarchitecture of the native dermal matrix of the bovine dermis, wherein the graft is non-crosslinked.