US 11,882,823 B2 · Composition and method of use of the same for preserving cells for analysis

Abstract

Stabilizing compositions for stabilizing a post-draw, but pre-analysis sample include, a saccharide, at least one heavy metal salt, and a pH from 5.9 to 8.0. The stabilizing compositions may include an aliphatic aldehyde, a buffer, and a preservative. The stabilizing compositions stabilize a sample for analysis. The analysis preformed on the stabilized cell may determine the state of a condition of interest, quantification of absolute cell counts, cellular activity, and qualitative analysis of cell types. Stabilizing a sample means that cells of the sample retain their biophysical properties, including biophysical properties of cell surface markers, for analysis. Preferably, the stabilizing compositions and methods may stabilize a sample for at least 16 days, and up to 30 days. The stabilizing compositions and methods may stabilize a sample for up to 180 days.

Claims8

Dependent claims

A method for stabilizing a biological specimen post-draw and prior to analysis, the method comprising:

contacting a biological specimen having cells of hematopoietic or non-hematopoietic origin and of a volume sufficient to conduct flow cytometry with a stabilizing composition, the stabilizing composition comprising:
a heavy metal salt,
saccharide having a concentration in the stabilizing composition from 1.5 to 3.0 Molar,
an aliphatic aldehyde having a concentration in the stabilizing composition from 0.001 to 0.15 Molar,
a pH from 5.9 to 8.0, and
a solvent;
storing the biological specimen contacted with the stabilizing composition at a temperature from minus 20 degrees Celsius to minus 200 degrees Celsius for at least 1 day to stabilize the biological specimen.

4 more elements

A method for stabilizing and analyzing a biological specimen post-draw, the method comprising:

contacting a biological specimen having cells of hematopoietic or non-hematopoietic origin and of a volume sufficient to conduct flow cytometry with a stabilizing composition, the stabilizing composition comprising:
a heavy metal salt,
saccharide having a concentration in the stabilizing composition from 1.5 to 3.0 Molar,
an aliphatic aldehyde having a concentration in the stabilizing composition from 0.001 to 0.15 Molar,
a pH from 5.9 to 8.0, and
a solvent;
storing the biological specimen contacted with the stabilizing composition at a temperature from minus 20 degrees Celsius to minus 200 degrees Celsius for at least 1 day to stabilize the biological specimen;
thawing the biological specimen contacted with the stabilizing composition;
staining the biological specimen contacted with the stabilizing composition;
lysing red blood cells in the biological specimen contacted with the stabilizing composition;
analyzing the biological specimen to determine the presence or absence of a condition of interest.

8 more elements

Dependencies are inferred from the claim text.

Composition and method of use of the same for preserving cells for analysis

Life of this patent

Composition and method of use of the same for preserving cells for analysis

Life of this patent

Abstract

Claims8