Integrated machines and methods for performing fully-automated biological evaluation and chemical analysis

An integrated machine for performing fully-automated biological evaluation and chemical analysis, comprising:

• a pretreatment module configured for enriching, concentrating and purifying a sample to be analyzed to provide a mother liquor to be analyzed;
• a component separation module, wherein an inlet of the component separation module is connected with the pretreatment module, and an outlet of the component separation module is connected with an inlet of a three-way diverter valve, and wherein the component separation module is configured to perform a separation of multiple compounds in the mother liquor to be analyzed, to produce a separated liquid effluent;
• a monitoring and identifying module, wherein an inlet of the monitoring and identifying module is connected with a first outlet of the three-way diverter valve, and wherein the monitoring and identifying module is configured for monitoring and collecting chromatographic signals of the separated liquid effluent in real time, as well as quantitative detection of suspicious compounds;
• a component collection module, wherein an inlet of the component collection module is connected with a second outlet of the three-way diverter valve, and an outlet of the component collection module is split into two paths, wherein an outlet of a first path is connected with the component separation module, and wherein the component collection module is configured for collecting, transferring and dissolving, redissolving, and pipetting the separated liquid effluent;
• a biological evaluation module connected with an outlet of a second path of the component collection module and configured to perform cell culture and to detect cytotoxic effect and toxic targets;
• a data processing and automated control module electrically connected with the monitoring and identifying module and the biological evaluation module, respectively, and configured to acquire, arrange and analyze the integrated data;
• wherein the component separation module comprises a high-pressure liquid pump, a sample injector, a chromatographic column for analysis, a chromatographic column for preparation, and a column oven;
• wherein, the mother liquor to be analyzed is concentrated and purified by the pretreatment module, and the high-pressure liquid pump and the sample injector are configured for introducing the mother liquor to be analyzed into the component separation module, the chromatographic column for analysis or the chromatographic column for preparation is configured to provide crude separation of the introduced mother liquor to be analyzed, and the column oven is configured for providing a constant column temperature during separation;
• wherein the monitoring and identifying module comprises an ultraviolet detector in tandem with a high-resolution mass spectrometer detector, and the separated liquid effluent introduced into the monitoring and identifying module sequentially passes through the ultraviolet detector and the high-resolution mass spectrometer detector to provide a spectrogram of chromatography-mass spectrometry of the separated liquid effluent;
• and
• wherein the component collection module comprises a plate storing scaffold, a mechanical arm, a fraction collector, a nitrogen blower, a pipetting workstation, and a mounting scaffold;
• wherein, the plate storing scaffold is arranged on a first side of the mounting scaffold and configured for storing plates;
• the mechanical arm is mounted at the top of the mounting scaffold and configured for transferring one plate of the plates at a time;
• the fraction collector is mounted on a second side of the mounting scaffold and connected with the second outlet of the three-way diverter valve and configured for collecting the separated liquid effluent split and flowed from the component separation module into the plate;
• the nitrogen blower is mounted on a third side of the mounting scaffold and configured for nitrogen blowing the separated liquid effluent transferred by the fraction collector in the plate to dryness;
• the pipetting workstation is mounted on a fourth side of the mounting scaffold and configured for preforming gradient dilution of a sample or positive control mother liquor and solution preparation.