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US 11,879,147 B2Granted
Chemoenzymatic synthesis of trehalose analogues
AssigneesCentral Michigan University, University of Maine System
InventorsBenjamin M. Swarts, Peter Woodruff
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Abstract
The present invention provides methods of synthesizing trehalose analogues; methods of detecting mycobacteria, and trehalose analogues.
Claims21
A method for synthesizing a trehalose analogue comprising:
- contacting a glucose analogue of Formula (I) with a monosaccharide donor, a magnesium salt, and a trehalose synthase from Thermoproteus tenax,
- wherein
- X is O;
- R1, R2 and R3 are independently selected from H, fluoro, —OR, and —N3;
- R4 is OH;
- and
- each R is independently selected from H and C1-6 alkyl;
- with the proviso that either the glucose analogue is not glucose or the monosaccharide donor is not a glucose donor.
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A method of preparing a trehalose analogue of formula
- comprising:
- reacting a compound of formula
- with UDP-glucose, a magnesium salt, and a trehalose synthase from Thermoproteus tenax, wherein
- each R is independently OH, F, H, or N3;
- X is O;
- and
- is not glucose.
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Dependencies are inferred from the claim text.