Compositions and methods for producing tobacco plants and products having reduced or eliminated suckers
AssigneeAltria Client Services LLC
InventorsDongmei Xu, Jesse Frederick, Chengalrayan Kudithipudi, Yanxin Shen, James Strickland, Jaemo Yang
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Abstract
The present disclosure provides the identification of genes involved in sucker growth in tobacco. Also provided are promoters that are preferentially active in tobacco axillary buds. Also provided are modified tobacco plants comprising reduced or no sucker growth. Also provided are methods and compositions for producing modified tobacco plants comprising reduced or no sucker growth.
Claims20
A method of producing a tobacco plant, said method comprising:
- (a) introducing a recombinant DNA construct comprising a promoter comprising a nucleic acid sequence at least 90% identical to a sequence selected from the group consisting of SEQ ID NOs:
- 148 and 157 operably linked to a transgene encoding a polypeptide at least 90% identical to the amino acid sequence of SEQ ID NO:
- 80 into a tobacco cell;
- and (b) generating said tobacco plant from said tobacco cell, wherein said tobacco plant comprises said recombinant DNA construct.
A method of introgressing a transgene into a tobacco variety, the method comprising:
- (a) crossing a first tobacco variety comprising a transgene encoding a polypeptide at least 90% identical to the amino acid sequence of SEQ ID NO:
- 80 operably linked to a promoter comprising a nucleic acid sequence at least 90% identical to a sequence selected from the group consisting of SEQ ID NOs:
- 148 and 157 with a second tobacco variety without said transgene to produce one or more progeny tobacco plants;
- and (b) selecting one of said one or more progeny tobacco plants, wherein said one or more progeny tobacco plants comprises said transgene.
Dependencies are inferred from the claim text.