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US 11,874,279 B2Granted
Methods and arrays for target analyte detection and determination of target analyte concentration in solution
AssigneeTrustees of Tufts College
InventorsDavid R. Walt, David M. Rissin
Life of this patent
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Abstract
Arrays of single molecules and methods of producing an array of single molecules are described. Arrays with defined volumes between 10 attoliters and 50 picoliters enable single molecule detection and quantitation.
Claims24
A system for determining a concentration of a target analyte in a sample, comprising:
- an optical detection system configured to identify optical signatures at individual assay locations on a substrate;
- and
- a computer that executes imaging processing software configured to process information from the optical detection system and to:
- perform a binary readout method to determine a percentage of a total number of the assay locations at which an optical detectable signal is observed by the optical detection system;
- and
- correlate the percentage of assay locations at which the detectable signal is present to a bulk concentration of the target analyte in the sample.
3 more elements
Dependencies are inferred from the claim text.