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US 11,873,524 B2Granted
Methods and systems for the rapid detection of Salmonella using infectious agents
AssigneeLaboratory Corporation of America Holdings
InventorsStephen Erickson, Jose S. Gil, Minh Mindy Bao Nguyen, Dwight Lyman Anderson, Jessica Stach
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Abstract
Disclosed herein are methods and systems for rapid detection of microorganisms in a sample. A genetically modified bacteriophage is also disclosed which comprises an indicator gene in the late gene region. The specificity of the bacteriophage, such as Salmonella-specific bacteriophage, allows detection of a specific microorganism, such as Salmonella spp. and an indicator signal may be amplified to optimize assay sensitivity.
Claims6
A method for detecting Salmonella spp. in a sample comprising:
- incubating a sample comprising powdered infant formula, for 30-120 minutes with a recombinant bacteriophage derived from a Salmonella-specific bacteriophage comprising, a genetic construct inserted into a late gene region of the bacteriophage genome, the genetic construct comprising:
- (i) an indicator gene;
- and
- (ii) a promoter controlling transcription of the indicator gene, wherein the promoter is a bacteriophage late promoter;
- and wherein the indicator gene is not contiguous with a gene encoding a structural bacteriophage protein and does not yield a fusion protein, and wherein expression of the indicator gene results in an indicator protein product;
- and
- detecting the indicator protein product, wherein positive detection of the indicator protein product indicates that Salmonella spp. is present in the sample.
4 more elements
Dependencies are inferred from the claim text.