Aconitic acid exporter (aexA) increases organic acid production in Aspergillus
AssigneeBattelle Memorial Institute
InventorsShuang Deng, Jon K. Magnuson, Joonhoon Kim, Kyle R. Pomraning, Ziyu Dai, Beth A. Hofstad
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Abstract
Recombinant Aspergillus genetically modified to increase expression of g8846, renamed herein as aconitic acid exporter (aexA), are provided, which in some examples are also genetically inactivated for an endogenous cis-aconitic acid decarboxylase (cadA) gene. Such recombinant Aspergillus produce more aconitic acid as compared to native Aspergillus. Also provided are methods of using such recombinant Aspergillus to increase production of aconitic acid and other organic acids, such as citric acid, itaconic acid, and 3-hydroxypropionic acid (3-HP).
Claims25
An isolated recombinant Aspergillus fungus comprising an exogenous nucleic acid molecule encoding an aconitic acid exporter (aexA) protein comprising at least 60% sequence identity to SEQ ID NO:
- 2, 3, 4, or 5, operably linked to an exogenous promoter, thereby overexpressing the aexA in the fungus.
An isolated nucleic acid molecule encoding an aconitic acid exporter (aexA) operably linked to a heterologous promoter.
Dependencies are inferred from the claim text.