Back to results
US 11,873,498 B2Granted
Tissue specific reduction of lignin
AssigneeThe Regents of the University of California
InventorsDominique Loque, Aymerick Eudes
Life of this patent
Estimating the term…
Abstract
The present invention provides an expression cassette comprising a polynucleotide that encodes a protein that diverts a monolignol precursor from a lignin biosynthesis pathway in the plant, which is operably linked to a heterologous promoter. Also provided are methods of engineering a plant having reduced lignin content, as well as plant cells, plant parts, and plant tissues from such engineered plants.
Claims6
A method of engineering a plant having reduced lignin content, the method comprising:
- introducing into the plant an expression cassette comprising a polynucleotide that encodes a protein that reduces the amount of coumaroyl-CoA, caffeoyl-CoA, and/or feruloyl-CoA that is available for a lignin biosynthesis pathway in the plant, wherein the protein is a 2-oxoglutarate-dependent dioxygenase (C2′H) having at least 95% amino acid sequence identity to SEQ ID NO:14;
- or the protein is a curcuminoid synthase having at least 95% amino acid sequence identity to SEQ ID NO:34 or SEQ ID NO:35;
- and further, wherein the polynucleotide is operably linked to a heterologous secondary cell wall-specific promoter, a fiber cell-specific promoter, or a promoter from a gene in the lignin biosynthesis pathway;
- and
- culturing the plant under conditions in which the protein is expressed.
2 more elements
Dependencies are inferred from the claim text.