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AssigneeThe Florida State University Research Foundation, Inc.
InventorsTeng Ma, Ang-Chen Tsai, Xuegang Yuan
Estimating the term…
The subject invention concerns materials and methods for culture of cell aggregates. The subject invention utilizes three-dimensional (3-D) inserts comprising micro-channels having selected dimensions. The inserts are provided in or on tissue culture plates that can be supported on a programmable rocking platform/station, thereby providing for a hydrodynamic environment that promotes 3-D aggregation of cells cultured on the plates. The supporting rocker is programmed to provide motion that generates hydrodynamic conditions that support 3-D cell aggregation and long-term culture. The subject invention also concerns an apparatus comprising a tissue culture vessel that comprises a 3-D insert of the present invention, and a programmable rocking platform/station that can provide motion to the vessel provided thereon, thereby generating hydrodynamic conditions and wave motion that support 3-D cell aggregation and cell culture. The subject invention also concerns methods for growing 3-D aggregates of cells.
A method for growing cells into aggregates of cells comprising culturing a cell in a micro-channel of a 3-D tissue culture insert provided in a tissue culture vessel, wherein said tissue culture vessel comprises one or more 3-D tissue culture inserts comprising one or more micro-channels that provide for a confined space for cells that are cultured in said one or more micro-channels and suitable culture media for culturing said cell, wherein said one or more micro-channels have a width of about 1 mm to about 5 mm, a length of about 20 mm to about 60 mm, and a height of about 5 mm to about 15 mm.
Dependencies are inferred from the claim text.