Antigen-binding chimeric proteins and methods and uses thereof
AssigneesVib Vzw, Vrij Universiteit Brussel
InventorsJan Steyaert, Els Pardon, Tomasz Uchanski, Wim Vranken
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Abstract
The present invention relates to the field of structural biology. More specifically, the present invention relates to novel antigen-binding chimeric proteins, their uses and methods in three-dimensional structural analysis of macromolecules, such as X-ray crystallography and high-resolution Cryo-EM, and their use as a therapeutic, diagnostic, or imaging tool. Even more specifically, the invention relates to a fusion of a scaffold protein and an antigen-binding domain wherein the scaffold protein of said fusion interrupts the Immunoglobulin domain topology to form a rigid chimer.
Claims22
An antigen-binding chimeric protein comprising an antigen-binding domain of an immunoglobulin fused with a scaffold protein, wherein the scaffold protein interrupts the primary topology of the antigen-binding domain at one or more exposed regions comprising a loop or β-turn of the antigen-binding domain, wherein said one or more exposed regions are not in complementarity-determining regions, wherein the antigen-binding domain retains the antigen-binding capacity, and wherein the scaffold protein is fused to the antigen-binding domain via at least two direct fusions or fusions made by a linker.
Dependencies are inferred from the claim text.