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US 11,872,551 B2Granted
Multiplexed polymerase chain reaction in micropipette format
OwnerAndrew Brodskyinventor
Life of this patent
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Abstract
A system may include a horizontal actuator to move a tray, to which a microwell plate and a microfluidic chip may be coupled. The system may include a vertical actuator to move a support arm, to which a plurality of pipettes or pipette tips may be coupled. The system may include a rotational actuator to move an angle bracket, to which a magnet may be coupled. The system may include a heater, through which the pipettes may extend. The system may include a pump to control the flow of fluids through the pipettes. Disclosed methods include performing PCR within the described system.
Claims19
A method, comprising:
- operating a pump to draw a biological sample from a well of a well plate into a pipette through a pipette tip of the pipette;
- operating a first actuator to move a first valve to a closed position to seal the biological sample within the pipette from the pipette tip;
- operating the pump to apply a positive gage pressure to the biological sample within the pipette;
- operating a second actuator to move a second valve to a closed position to seal the biological sample within the pipette from the pump;
- and
- operating a heater to heat the biological sample within the pipette, wherein a polymerase chain reaction occurs within the heated biological sample within the pipette between the first valve and the second valve.
3 more elements
A method, comprising:
- operating a pump to draw a biological sample from a well of a well plate into a first pipette conduit through a first pipette tip;
- operating a first actuator to move a first valve to a closed position to seal the biological sample within the first pipette conduit from the first pipette tip;
- operating the pump to apply a positive gage pressure to the biological sample within the first pipette conduit;
- operating a second actuator to move a second valve to a closed position to seal the biological sample within the first pipette conduit from the pump;
- and
- operating a heater to heat the biological sample within the first pipette conduit, wherein a chemical reaction occurs within the heated biological sample within the first pipette conduit between the first valve and the second valve.
3 more elements
Dependencies are inferred from the claim text.