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US 11,871,771 B2Granted
Glucosyl Stevia composition
AssigneePureCircle Sdn Bhd
InventorAvetik Markosyan
Life of this patent
Estimating the term…
Abstract
Glucosyl Stevia compositions are prepared from steviol glycosides of Stevia rebaudiana Bertoni. The glucosylation was performed by cyclodextrin glucanotransferase using the starch as source of glucose residues. The glucosyl Stevia compositions were purified to >95% content of total steviol glycosides. The compositions can be used as sweetness enhancers, flavors, flavor enhancers and sweeteners in foods, beverages, cosmetics and pharmaceuticals.
Claims25
A process for producing a glucosyl stevia composition, comprising the steps of:
- adding starch into water to form a starch suspension;
- adding a mixture of a-amylase and cyclodextrin glucanotransferase (CGTase) into the starch suspension and incubating for about 0.5 to 2 hours at about 75-80° C., resulting in a liquefied starch suspension;
- inactivating the α-amylase by low pH heat treatment;
- adding steviol glycosides into the liquefied starch suspension, resulting in a first reaction mixture;
- and
- adding a second batch of CGTase into the first reaction mixture and incubating for about 24-48 hours at about 65° C.,
- adding soybean ß-amylase, resulting in a second reaction mixture;
- and
- incubating the second reaction mixture for 12-16 hours at 35-55° C.;
- wherein:
- the glucosyl stevia composition comprises steviol glycoside derivatives having twenty or less α-1,4-glucosyl residues, and
- the glucosyl stevia composition comprises greater than 25% by weight di-, tri-, and tetraglucosyl Rebaudioside A and greater than about 9% by weight tri- and tetraglucosyl steviosides.
9 more elements
Dependencies are inferred from the claim text.