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US 11,866,779 B2Granted
Jettison-MS for nucleic acid species
AssigneeNortheastern University
InventorsRoger W. Giese, Poguang Wang
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Abstract
MALDI-MS operated slightly under matrix storm conditions with a high-energy-transfer, acidic matrix, or these conditions with a high sample to matrix ratio, can with ultrasensitivity detect a nucleobase, modified or canonical, of a nucleic acid species as a jettisoned, protonated molecule.
Claims20
A mass spectrometry method for detecting a modified nucleobase in a nucleic acid species, comprising
- a) providing a sample, wherein the sample comprises:
- (i) a modified nucleobase attached in N-glycosidic linkage to a ribose or deoxyribose moiety of a nucleic acid, nucleoside, nucleotide or oligonucleotide;
- and
- (ii) a matrix, wherein the matrix comprises at least one Brønsted acidic proton source;
- and
- b) subjecting the sample to at least one laser pulse, wherein the laser fluence is about 70% to about 95% of the matrix storm level.
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A mass spectrometry method for detecting a canonical nucleobase in a nucleic acid species, comprising
- a) providing a sample, wherein the sample comprises:
- (i) a canonical nucleobase attached in N-glycosidic linkage to a ribose or deoxyribose moiety of a nucleic acid, nucleoside, nucleotide or oligonucleotide;
- (ii) a matrix, wherein the matrix comprises at least one Bøonsted acidic proton source;
- and
- (iii) the molar ratio of the overall nucleobases in the sample to the matrix is about 0.03 to about 0.3;
- and
- b) subjecting the sample to at least one laser pulse, wherein the laser fluence is about 70% to about 95% of the matrix storm level.
4 more elements
Dependencies are inferred from the claim text.