Fibrosis-inhibiting composition, cells producing same, and cell sheet comprising said cells
AssigneesKanonCure, Inc., National University Corporation Tottori University
InventorsYohei Kono, Noriko Itaba, Goshi Shiota
Life of this patent
Estimating the term…
Abstract
[Problem] To provide a composition and a cell sheet which are highly effective for inhibiting fibrosis, or cells having fibrosis-inhibiting activity, which are useful in regenerative medicine. [Solution] A medium containing IC-2 or a related compound is inoculated with mesenchymal stem cells or bone marrow mononuclear cells, and the cells are cultured for a prescribed period of time while the IC-2 or related compound is maintained at a constant concentration, thereby allowing a composition and a cell sheet which are highly effective for inhibiting fibrosis, or cultured cells having fibrosis-inhibiting activity, to be obtained.
Claims6
A method of producing a composition having high fibrosis inhibitory effect comprising the steps of:
- i) culturing bone marrow mononuclear cells or mesenchymal stem cells in a culture medium containing IC-2 or an analogous compound thereof for at least 9 days, characterized in that the concentration of IC-2 or the analogous compound thereof is maintained throughout the cultivation;
- ii) collecting cultured cells, secreted products of the cells or both from the culture obtained in the step i);
- and
- iii) recovering proteins from the cultured cells, the secreted products of the cells or the both collected in the step ii)
- wherein said composition comprises MMP-1, MMP-14, MMP-2, MMP-13, and thioredoxin (TRX).
A method of producing a cell sheet having high fibrosis inhibitory effect comprising the steps of:
- i) culturing mesenchymal stem cells in a culture medium containing IC-2 or an analogous compound thereof for at least 9 days, characterized in that the concentration of IC-2 or the analogous thereof is maintained throughout the cultivation;
- and
- ii) collecting a cell sheet containing the mesenchymal stem cells and secreted products of said cells generated in the step i)
- wherein said cell sheet comprises MMP-1, MMP-14, MMP-2, MMP-13, and thioredoxin (TRX).
A method for increasing production of a fibrosis inhibitor by a mesenchymal stem cell comprising culturing bone marrow mononuclear cells or mesenchymal stem cells in a culture medium containing IC-2 or an analogous compound thereof for at least 9 days, characterized in that the concentration of IC-2 or the analogous compound thereof is maintained throughout the cultivation, wherein the fibrosis inhibitor is selected from the group consisting of MMP-1, MMP-14, MMP-2, MMP-13, thioredoxin (TRX), and a combination thereof.
Dependencies are inferred from the claim text.