Compositions and methods for enhanced fluorescence
AssigneesLife Technologies Corporation, Pierce Biotechnology, Inc.
InventorsSurbhi Desai, Marie Christine Nlend, Kyle Gee, Matthew Baker, Robert Aggeler, Scott Sweeney, Aleksey Rukavishnikov, Shih-Jung Huang
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Abstract
This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.
Claims14
A composition, comprising a first conjugated antibody comprising two to ten fluorescent labels and two or more spacer molecules covalently attached to a first antibody, wherein the fluorescent labels and spacer molecules are not covalently attached to each other and wherein the composition exhibits increased fluorescence relative to a composition comprising a second conjugated antibody that is the same as and has a same degree of fluorescent labeling as the first conjugated antibody but that is lacking spacer molecules covalently attached to the second conjugated antibody, wherein the spacer molecules, which are covalently attached to the first antibody, are selected from sulfo-NHS-acetate, MS(PEG)4, or NHS-Betaine.
Dependencies are inferred from the claim text.