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US 11,863,041 B2Granted
Devices and methods for clearing and molecular labeling of intact tissues
AssigneeUniversity of Massachusetts
InventorsJoseph Fossland Bergan, Joseph Frank Dantas Dwyer
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Abstract
The invention provides devices and methods for moving charged molecules into and out of tissue samples. This invention is particularly useful for removing endogenous heterogenous particles from tissue samples and for introducing exogenous charged molecules (e.g., antibodies, dyes) into tissue samples.
Claims5
A method for tissue delipidation, comprising:
- providing a whole adult mouse brain submerged in an electrically conducting solution comprising NaOH, LiOH and H3BO3, or a salt thereof, in a cylindrically shaped channel having a longitudinal dimension, wherein the whole adult mouse brain is positioned stationary to the cylindrically shaped channel;
- and
- applying an electric field within the cylindrically shaped channel and simultaneously applying a magnetic field within the cylindrically shaped channel and perpendicular to the electric field, thereby inducing lipid particles in the whole adult mouse brain to flow in a direction perpendicular to both the electric field and the magnetic field, wherein delipidation is completed in about 15 hours,
- wherein
- the electrically conducting solution comprises a polar solvent and an electrolyte;
- and
- the electric field and the magnetic field are both perpendicular to the longitudinal dimension and jointly induce charged lipid particles in the whole adult mouse brain to flow in a direction perpendicular to both the electric field and the magnetic field.
4 more elements
Dependencies are inferred from the claim text.