Method for detecting L-serine based on cysteine desulfurase-containing living Escherichia coli cell

Abstract

The present disclosure provides a method for detecting L-serine based on cysteine desulfurase-containing living Escherichia coli cells, and belongs to the technical field of amino acid detection. The method includes the following steps: incubating an unknown sample with the cysteine desulfurase-containing living E. coli cells to produce a red substance, and qualitatively or semi-quantitatively detecting L-serine content in the unknown sample according to color changes of the red substance of the living E. coli cells, or quantitatively detecting L-serine content in the unknown sample by measuring absorbance of a lysate of the living E. coli cells. The detection method provided by the present disclosure is simple and convenient in process, few in reaction steps and stable in enzymatic activity of living cells.

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