Compositions and methods comprising the use of a Bacillus agaradhaerens inulosucrase (INUO)
AssigneeDanisco US Inc.
InventorsSlavko Kralj, Marc Kolkman, Chris Leeflang, Johannes G. De Nobel, Arjen Hoekstra, Veli Alkan
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Abstract
Bacillus agaradhaerens strain WDG185 expresses an inulosucrase that efficiently synthesizes a broad range of IOS with a GF range of GF3-GF30. The isolated and/or purified inulosucrase, recombinantly engineered variants thereof, active fragments thereof, synthetic nucleic acids encoding the inulosucrase, its variants, or its active fragments, host cells comprising the synthetic nucleic acids, and compositions comprising the inulosucrase are provided. Methods of using the compositions include the manufacture of inulooligosaccharides.
Claims20
A method of producing an inulosaccharide product, comprising:
- providing an inulosucrase by expressing a recombinant nucleic acid encoding the inulosucrase from a host cell, wherein the recombinant nucleic acid is heterologous to the host cell, and
- contacting the inulosucrase with a fructose source to produce the inulosaccharide product,
- wherein the inulosucrase (i) comprises an amino acid sequence that is at least 90% identical to amino acids 32-453 of SEQ ID NO:
- 4, and (ii) catalyzes polymerization of inulin oligosaccharides containing β-(2→1) linkages.
A method of producing a tailored oligofructoside product, comprising:
- providing an inulosucrase by expressing a recombinant nucleic acid encoding the inulosucrase from a host cell, wherein the recombinant nucleic acid is heterologous to the host cell, and
- contacting the inulosucrase with a sucrose analogue having the glucose cap of sucrose substituted by another saccharide to produce the tailored oligofructoside product,
- wherein the inulosucrase (i) comprises an amino acid sequence that is at least 90% identical to amino acids 32-453 of SEQ ID NO:
- 4, and (ii) catalyzes polymerization of inulin oligosaccharides containing β-(2→1) linkages,
- wherein the sucrose analogue has the glucose cap of sucrose substituted by a galactose, a mannose, a fucose, or a xylose.
Dependencies are inferred from the claim text.