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US 11,859,213 B2Granted
Development of superior chimerism by hiPSC engineering and embryo aggregation
AssigneeRegents of the University of Minnesota
InventorsDaniel J. Garry, Mary G. Garry, Geunho Maeng, Ohad Gafni
Life of this patent
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Abstract
Provided herein are method to increase the efficiency of interspecies chimera generation.
Claims12
A method to increase the efficiency of human:
- non-human animal chimera generation comprising introducing one or more human stem cells into a non-human embryo, wherein multiple embryos are dissociated and the dissociated aggregate is layered with one or more human cells and cultured prior to transfer into a synchronized surrogate, wherein the aggregated embryo and cells results in increased efficiency of chimera generation, further comprising knocking down or out the expression of TP53 in the one or more human cells, wherein the one or more human cells are induced human pluripotent stem cells (iPSCs).
A method to increase the efficiency of human:non-human animal chimera generation comprising introducing one or more human cells into a non-human embryo, wherein the one or more human cells has reduced expression of TP53;
- wherein the reduced expression of TP53 results in the increased efficiency of chimera generation as compared to generating a human:
- non-human animal chimera with one or more human cells with wild-type expression of TP53.
Dependencies are inferred from the claim text.