Elastomeric proteins
AssigneeBolt Threads, Inc.
InventorsJoshua Kittleson, Michael Lee, David N. Breslauer, Daniel M. Widmaier
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Abstract
This invention relates to elastomeric protein and elastomeric protein production. In particular, the invention is directed to elastomeric protein sequences, including methods and compositions for production of elastomeric protein sequences, such as expression constructs, and host cells, and including compositions generated from the elastomeric protein sequences.
Claims21
A method for producing a composition comprising a recombinant resilin protein, comprising:
- culturing a population of recombinant yeast host cells in a fermentation, wherein said recombinant yeast host cells comprise a vector comprising a secreted resilin coding sequence selected from the group consisting of SEQ ID NOs:
- 2, 3, 5, 6, 7, 8, 9, 10, 11, and 12, and wherein said recombinant yeast host cells secrete a recombinant resilin protein encoded by said secreted resilin coding sequence;
- and
- purifying said recombinant resilin protein from said fermentation.
A vector comprising a secreted resilin coding sequence selected from the group consisting of SEQ ID NOs:
- 2, 3, 5, 6, 7, 8, 9, 10, 11, and 12.
A composition comprising a gel comprising a recombinant resilin selected from the group consisting of SEQ ID NOs:
- 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12.
Dependencies are inferred from the claim text.