Compositions and methods for enhanced production of enduracidin in a genetically engineered strain of streptomyces fungicidicus
AssigneeOregon State University
InventorsT. Mark Zabriskie, Xihou Yin
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Abstract
This disclosure describes compositions and methods for enhanced production of enduracidin in genetically engineered strains of Streptomyces fungicidicus. In particular, the present disclosure describes the genetic manipulation of regulatory genes orf24 and orf18 associated with the enduracidin (enramycin) biosynthesis gene cluster from Streptomyces fungicidicus to generate vector constructs and recombinant strains producing greater yields of enduracidin.
Claims21
A recombinant strain of Streptomyces fungicidicus, comprising an augmented open reading frame-24 (orf24) that encodes the amino acid sequence of SEQ ID NO:
- 26 or a diminished open reading frame-18 (orf18), wherein an enhanced production of enduracidin is obtained with the recombinant strain of Streptomyces fungicidicus in comparison to that obtained with a control strain of Streptomyces fungicidicus.
An expression vector comprising an augmented open reading frame orf24 (SEQ ID NO:
- 38).
A disruption vector comprising an open reading frame orf18 (SEQ ID NO:
- 37) which has been nulled.
Dependencies are inferred from the claim text.