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US 11,857,643 B2Granted
Compositions and methods for enhanced fluorescence
AssigneesLife Technologies Corporation, Pierce Biotechnology, Inc.
InventorsSurbhi Desai, Marie Christine Nlend, Kyle Gee, Matthew Baker, Robert Aggeler, Scott Sweeney, Aleksey Rukavishnikov, Shih-Jung Huang
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Abstract
This disclosure relates to the field of fluorescent dyes, and in particular, compositions and methods for increasing fluorescent signals and the reduction of fluorescent quenching.
Claims11
A method for increasing fluorescence of a first fluorescently labeled antibody, the method comprising:
- (a) conjugating two or more spacer molecules to a first antibody;
- and
- (b) conjugating from two to ten fluorescent labels to the first antibody;
- wherein steps (a) and (b) are done simultaneously or sequentially in any order to form the first fluorescently labeled antibody;
- wherein the spacer molecules and fluorescent labels are not conjugated to each other;
- wherein the first fluorescently labeled antibody has a fluorescence that is greater than a fluorescence of a second fluorescently labeled antibody that comprises a second antibody that is the same as the first antibody and has the same number and type of fluorescent labels conjugated thereto, but does not include the spacer molecules conjugated to the second antibody;
- and
- wherein the spacer molecules are selected from sulfo-NHS-acetate, MS(PEG)4, or NHS-Betaine.
5 more elements
Dependencies are inferred from the claim text.