Recombinant measles virus expressing zika virus prM and E proteins

A nucleic acid construct which comprises:

• (1) a polynucleotide encoding at least (i) the precursor of membrane (prM) protein of a Zika virus (ZIKV), and the envelope (E) protein of a ZIKV or a truncated version thereof of SEQ ID NO:
• 26, SEQ ID NO:
• 29 and SEQ ID NO:
• 32, or (ii) the E protein of a ZIKV or the truncated version thereof of SEQ ID NO:
• 26, SEQ ID NO:
• 29 and SEQ ID NO:
• 32;
• and
• (2) a cDNA molecule encoding a full-length, infectious antigenomic (+) RNA strand of a live-attenuated measles virus (MV) vaccine strain;
• wherein the polynucleotide encoding at least (i) the prM protein of the ZIKV, and the E protein of the ZIKV or the truncated version thereof, or (ii) the E protein of the ZIKV or the truncated version thereof and the cDNA molecule are operatively linked;
• the nucleic acid construct comprising the following polynucleotides from 5′ to 3′:
• (a) a polynucleotide encoding the N protein of the MV;
• (b) a polynucleotide encoding the P protein of the MV;
• (c) the polynucleotide encoding at least (i) the prM protein of the ZIKV, and the E protein of the ZIKV or the truncated version thereof, or (ii) the E protein of the ZIKV or the truncated version thereof;
• (d) a polynucleotide encoding the M protein of the MV;
• (e) a polynucleotide encoding the F protein of the MV;
• (f) a polynucleotide encoding the H protein of the MV;
• and
• (g) a polynucleotide encoding the L protein of the MV;
• wherein said polynucleotides are operably linked in the nucleic acid construct and under a control of viral replication and transcription regulatory sequences.