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US 11,857,576 B2Granted
Compositions and methods for repairing cartilage defects
AssigneeVericel Corporation
InventorsRoss A. Tubo, Xing Zhao
Life of this patent
Estimating the term…
Abstract
The present disclosure provides compositions and methods for repairing cartilage defects.
Claims19
A composition comprising:
- cultured allogeneic cells comprising chondrocytes grown from a cryogenically frozen cell bank sample;
- and
- a resorbable collagen membrane comprising a combination of type I and type Ill porcine collagen derived from porcine peritoneum;
- wherein the cultured allogeneic cells are seeded on the resorbable collagen membrane at a density of at least 250,000 cells per cm2,
- wherein the allogeneic cells are arranged in a monolayer on the resorbable collagen membrane, and
- wherein the allogeneic cells are dedifferentiated.
3 more elements
A method of manufacturing an allogeneic cartilage matrix, the method comprising steps of:
- thawing a cryogenically frozen cell bank sample comprising chondrocytes;
- culturing the cells of the sample;
- characterizing the sample;
- preparing a resorbable collagen membrane;
- seeding the resorbable collagen membrane with the cultured cells;
- and
- packaging the resorbable collagen membrane,
- wherein characterizing the sample comprises characterizing the sample by one or more control assays comprising:
- an agarose assay comprising an acceptance criterion comprising at least 6.8% of cells forming colonies for at least two divisions.
6 more elements
A composition comprising:
- cultured allogeneic cells comprising chondrocytes grown from a cryogenically frozen cell bank sample;
- and
- a resorbable collagen membrane comprising a combination of type I and type III porcine collagen derived from porcine peritoneum, the resorbable collagen membrane being coated with a fibrin adhesive;
- wherein the cultured allogeneic cells are seeded on the resorbable collagen membrane at a density of at least 250,000 cells per cm2,
- wherein the allogeneic cells are dedifferentiated, and
- wherein the composition comprises at least one of a chondrocyte marker and a non-chondrocyte marker.
3 more elements
Dependencies are inferred from the claim text.