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AssigneeThe Florida International University Board of Trustees
InventorsJin He, Santosh Khatri, Popular Pandey
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A system, apparatus, device and/or method for electrical label-free detection of single molecule via a glass or quartz nanopipette biosensor, one type of solid state nanopore biosensor based on resistive pulse measurements, are provided. The method of the subject invention effectively slows down the motion of biomolecules and concentrates them at the nanopipette tip. By driving biomolecules to come out of the nanopipette barrel and move in the fibrin hydrogel, unprecedented sensitivity using the typical size nanopipettes can be achieved. Small molecules such as doxorubicin, ATP, GTP, and short peptides can be easily detected with very high event rate, which greatly reduces the fabrication and measurement difficulties and open opportunities for various new applications for nanopipette biosensors.
A system for enhanced single molecule detection, the system comprising a nanopipette disposed with an electrode;
A method for detecting intermolecular interactions between small biomolecules, the method comprising introducing a sample solution containing the small biomolecules into an open barrel of a nanopipette, the nanopipette having a nanopore at the tip and a working electrode disposed within the sample solution containing the small biomolecules;
A method for slowing down the translocation of small biomolecules through the nanopore of a nanopipette, the method comprising introducing a sample solution containing the small biomolecules into an open barrel of a nanopipette, the nanopipette having a nanopore at the tip and a working electrode disposed within the sample solution containing the small biomolecules;
Dependencies are inferred from the claim text.