Fusion protein for remodeling antibody glycoform

A fusion protein comprising a fucosidase fused with either an N-terminal end or a C-terminal end of an endoglycosidase or a fragment or a mutant thereof having endoglycosidase activity;

• wherein the fusion protein exhibits both fucosidase activity and the endoglycosidase activity;
• wherein the fucosidase is selected from the group consisting of Lactobacillus casei α-L fucosidase C (Alfc), Bacteroides fragilis fucosidase (BF3242), Bacteroides thetaiotaomicron α-L-fucosidase (BT2970), Emticicia oligotrophica α-L-fucosidase (EO0918) and Elizabethkingia miricola α-(1-6) fucosidase (Emfuc3);
• and
• wherein the endoglycosidase is selected from the group consisting of Streptococcus pyogenes endoglycosidase S, a truncated fragment of Streptococcus pyogenes endoglycosidase S comprising an immunoglobulin G (IgG) binding domain thereof, a mutant of Streptococcus pyogenes endoglycosidase S having a mutation at amino acid position D233, wild type Streptococcus pyogenes endoglycosidase S2, a truncated fragment of Streptococcus pyogenes endoglycosidase S2 comprising an immunoglobulin G (IgG) binding domain thereof, and a mutant of Streptococcus pyogenes endoglycosidase S2 having a mutation at amino acid position T138, D182, D184, E186, D226, or T227, wherein the wild type Streptococcus pyogenes endoglycosidase S2 comprises the amino acid sequence of SEQ ID NO:
• 26 at positions 463 to 1307.