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US 11,851,410 B2Granted
Sensors for detection of negatively charged phosphate-containing membranes and membrane components
AssigneeThe Governing Council of the University of Toronto
InventorsPatrick Thomas Gunning, Dziyana Kraskouskaya, Aaron Cabral, Bronte Murcar-Evans, Krimo Toutah, Elvin De Araujo
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Abstract
This application relates to a method of detecting negatively charged phosphate-containing membranes and membrane components, such as in cell membranes or artificial lipid vesicles, and its use, for example, in detecting apoptosis and bacterial infection.
Claims11
A method of detecting negatively charged phosphate-containing membrane components comprising:
- (a) combining a solution of a sample suspected of comprising negatively charged phosphate-containing membrane components with a solution comprising a compound of Formula V, or a metal chelate salt thereof:
- wherein
- L2 is a linker group selected from C1-20alkylene, C2-20alkenylene, C2-20alkynylene and C3-10-cycloalkylene, or a combination thereof, each of which is optionally oxo-substituted (═O) 1-3 times, and in which 1-3 carbon atoms are optionally replaced with a heteromoiety selected from NH, O, S and Si;
- and
- R3 is selected from
- (b) detecting a fluorescence signal;
- wherein detection of the fluorescence signal in (b) indicates that the sample comprises negatively charged phosphate-containing membrane components.
4 more elements
A compound of Formula V, or a metal chelate salt thereof:
- wherein
- L2 is a linker group selected from C1-20alkylene, C2-20alkenylene, C2-20alkynylene and C3-10-cycloalkylene, or a combination thereof, each of which is optionally oxo-substituted (═O) 1-3 times, and in which 1-3 carbon atoms are optionally replaced with a heteromoiety selected from NH, O, S and Si;
- and
- R3 is selected from
1 more element
Dependencies are inferred from the claim text.