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US 11,845,946 B2Granted
Tobacco plants exhibiting altered photosynthesis and methods of making and using
AssigneeAltria Client Services LLC
InventorsYanxin Shen, Dongmei Xu
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Abstract
This disclosure provides tobacco plants that exhibit altered photosynthesis as well as methods of making and using such plants.
Claims20
A method of making a Nicotiana tabacum plant, comprising:
- selecting a N. tabacum plant comprising a non-natural mutation in an endogenous nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 21, 23, and 25;
- crossing or selfing said selected N. tabacum plant to produce at least one progeny seed;
- and
- obtaining at least one progeny N. tabacum plant grown from said at least one progeny seed, wherein said at least one progeny N. tabacum plant comprises said non-natural mutation;
- and
- wherein said at least one progeny N. tabacum plant comprising said non-natural mutation has reduced expression of a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 21, 23, and 25 compared to a control tobacco plant lacking said non-natural mutation.
5 more elements
A N. tabacum plant, or part thereof, comprising an altered photosynthesis phenotype and reduced activity of a polypeptide selected from the group consisting of SEQ ID NOs:
- 22, 24, and 26;
- wherein said altered photosynthesis phenotype is selected from the group consisting of a reduced concentration of thylakoid membranes in the photosystems, a reduced amount of total chlorophyll, and an increased ratio of chlorophyll a to chlorophyll b;
- wherein said reduced or increased amounts or ratio are in comparison to a N. tabacum plant having wild-type activity of a polypeptide selected from the group consisting of SEQ ID NOs:
- 22, 24, and 26.
1 more element
A N. tabacum plant, or part thereof, comprising a non-natural mutation in an endogenous nucleic acid, the wild type endogenous nucleic acid encoding a sequence selected from the group consisting of -SEQ ID NOs:
- 22, 24, and 26;
- wherein said N. tabacum plant comprising said non-natural mutation has reduced expression of a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
- 21, 23, and 25 compared to a control tobacco plant lacking said non-natural mutation.
Dependencies are inferred from the claim text.