Bladder cell formation control agent and plant body into which bladder cell formation control agent has been introduced
AssigneeMasashi Mori
InventorsMasashi Mori, Tomohiro Imamura, Hiroharu Mizukoshi, Kanako Nishizawa
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Abstract
A gene that controls epidermal bladder cell formation is isolated and identified. Specifically, a gene involved in an epidermal bladder cell formation control action is identified by: recognizing that a plant body with remarkably reduced epidermal bladder cells appears by performing EMS mutagenesis treatment on seeds; and comparing genomic DNA of the mutant and wild-type genomic DNA.
Claims4
A method of forming epidermal bladder cells in a plant body, including introducing one of a gene shown in any one of the following items (1) to (4) and a vector carrying the gene, or a protein having an epidermal bladder cell formation action, which has an amino acid sequence of any one of the following items (5) to (6), into a plant body of a genus Chenopodium, Spinacia, or Mesembryanthemum:
- (1) a gene encoding a polypeptide having the amino acid sequence of SEQ ID NO:
- 3;
- (2) a gene encoding a polypeptide that has 90% or more identity to the amino acid sequence set forth in SEQ ID NO:
- 3, and that has 50% or more of an epidermal bladder cell formation action compared to that of the amino acid sequence set forth in SEQ ID NO:
- 3;
- (3) a gene having the sequence set forth in SEQ ID NO:
- 4;
- (4) a gene that has 98% or more identity with DNA having the base sequence set forth in SEQ ID NO:
- 4, and that encodes a polypeptide having an epidermal bladder cell formation action;
- (5) the amino acid sequence of SEQ ID NO:
- 3;
- and
- (6) an amino acid sequence that has 90% or more identity to the amino acid sequence set forth in SEQ ID NO:
- 3, and that has 50% or more of an epidermal bladder cell formation action compared to that of the amino acid sequence set forth in SEQ ID NO:
- 3.
A plant body having enhanced expression of a protein having an epidermal bladder cell formation action, which has an amino acid sequence of any one of the following items (1) to (2):
- (1) the amino acid sequence of SEQ ID NO:
- 3;
- and
- (2) an amino acid sequence that has 90% or more identity to the amino acid sequence set forth in SEQ ID NO:
- 3, and that has 50% or more of an epidermal bladder cell formation action compared to that of the amino acid sequence set forth in SEQ ID NO:
- 3, wherein
- the plant body is of a genus Chenopodium, Spinacia, or Mesembryanthemum.
Dependencies are inferred from the claim text.