Analysis of chromatin using a nicking enzyme
AssigneeNew England Biolabs, Inc.
InventorsChaithanya Ponnaluri, Hang-Gyeong Chin, Pierre O. Esteve, Sriharsa Pradhan
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Abstract
Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.
Claims9
A method for detecting abnormal cells in or from a tissue section or biopsy wherein the abnormal cells have altered chromatin compared to normal cells, comprising:
- (a) reacting the cells with a composition comprising a mixture of a nicking enzyme, four dNTPs, and at least one labeled dNTP, and a polymerase to selectively label the chromatin;
- (b) incorporating the labeled dNTP into the chromatin of the cells to form labelled nucleic acid;
- and
- (c) analyzing the pattern of labelled nucleic acid in the cells.
Dependencies are inferred from the claim text.