Compositions and methods for promoting hemostasis and other physiological activities

A method for preventing the movement of a bodily fluid or contaminant on or in a subject, comprising applying a formulation in a dosage form, the formulation comprising self-assembling peptides forming an ionically crosslinked barrier structure when applied to a surgical incision or wound site on or in the subject, wherein the self-assembling peptides consist of from 8 to 32 amino acid residues and consist of one or more amino acid residues selected from the group consisting of Formulas I-IV:

• ((Xaaneu-Xaa+)x(Xaaneu-Xaa−)y)n (I)
• ((Xaaneu-Xaa−)x(Xaaneu-Xaa+)y)n (II)
• ((Xaa+-Xaaneu)x(Xaa−Xaaneu)y)n (III)
• ((Xaa−-Xaaneu)x(Xaa+Xaaneu)y)n (IV)
• wherein Xaaneu is alanine, valine, glycine, isoleucine, phenylalanine, tyrosine or leucine;
• Xaa+ is arginine, lysine or ornithine;
• Xaa− is aspartic acid or glutamic acid;
• x and y are integers having a value of 1 or 2, or 4, independently;
• and n is an integer having a value of 1-4;
• wherein the self-assembling peptides are present in an aqueous solution in the dosage form in a concentration between 1.0% weight to volume and 4.0% weight to volume, inclusive, forming an ionically crosslinked self-assembled barrier structure upon contacting physiological fluids or tissues, which prevents passage of a bodily fluid or contaminant through the structure.

A formulation in a dosage form, the formulation forming an ionically crosslinked barrier structure for preventing the movement of bodily fluids and contaminants, comprising self-assembling peptides, wherein the self-assembling peptides consist of from 8 to 32 amino acid residues and consist of one or more amino acid residues selected from the group consisting of Formulas I-IV:

• ((Xaaneu-Xaa+)x(Xaaneu-Xaa−)y)n (I)
• ((Xaaneu-Xaa−)x(Xaaneu-Xaa+)y)n (II)
• ((Xaa+-Xaaneu)x(Xaa−Xaaneu)y)n (III)
• ((Xaa−-Xaaneu)x(Xaa+Xaaneu)y)n (IV)
• wherein Xaaneu is alanine, valine, glycine, isoleucine, phenylalanine, tyrosine or leucine;
• Xaa+ is arginine, lysine or ornithine;
• Xaa− is aspartic acid or glutamic acid;
• x and y are integers having a value of 1 or 2, or 4, independently;
• and n is an integer having a value of 1-4;
• wherein the self-assembling peptides are present in a powder, and when the powder is formulated into an aqueous solution in the dosage form in a concentration between 1.0% weight to volume and 4.0% weight to volume, inclusive, the aqueous solution in the dosage form forms an ionically crosslinked self-assembled barrier structure upon contacting physiological fluids or tissues, which prevents passage of a bodily fluid or contaminant through the structure.

A method for preventing the movement of a bodily fluid or contaminant on or in a subject, comprising applying a formulation in a dosage form, the formulation comprising self-assembling peptides forming an ionically crosslinked barrier structure when applied to a surgical incision or wound site on or in the subject, wherein the self-assembling peptides consist of from 8 to 32 amino acid residues and consist of one or more amino acid residues selected from the group consisting of Formulas I-IV:

• ((Xaaneu-Xaa+)x(Xaaneu-Xaa−)y)n (I)
• ((Xaaneu-Xaa−)x(Xaaneu-Xaa+)y)n (II)
• ((Xaa+-Xaaneu)x(Xaa−Xaaneu)y)n (III)
• ((Xaa−-Xaaneu)x(Xaa+Xaaneu)y)n (IV)
• wherein Xaaneu is alanine, valine, glycine, isoleucine, phenylalanine, tyrosine or leucine;
• Xaa+ is arginine, lysine or ornithine;
• Xaa− is aspartic acid or glutamic acid;
• x and y are integers having a value of 1 or 2, or 4, independently;
• and n is an integer having a value of 1-4;
• wherein the self-assembling peptides are present in a powder which is formulated into an aqueous solution in the dosage form, wherein the aqueous solution in the dosage form is in a concentration between 1.0% weight to volume and 4.0% weight to volume, inclusive, forming an ionically crosslinked self-assembled barrier structure upon contacting physiological fluids or tissues, which prevents passage of a bodily fluid or contaminant through the structure.