Composition comprising a bioactive molecule
AssigneeEucaryo Beheer B.V.
InventorWilhelmus Antonius Maria Van Lith
Life of this patent
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Abstract
A process for preparing a composition including a bioactive molecule including the steps of a. Dissolving a bioactive molecule having at least one acid group with a base at pH=>11 to obtain a solution A b. Neutralizing the solution A with an acid to obtain the composition including the bioactive molecule having a pH between 6 and 9, wherein PEG is added before the neutralizing step in case either the base and/or the acid is an inorganic compound, and to a composition including a high concentration of natamycin.
Claims15
A process for preparing a composition comprising a bioactive molecule comprising the steps of:
- a) dissolving a bioactive molecule having at least one acid group with an aqueous base at pH≥11 to obtain a solution A;
- and
- b) neutralizing the solution A with an acid to obtain the composition comprising the bioactive molecule having a pH between 6 and 9,
- wherein polyethylene glycol (PEG) is added at a temperature between 30° C. and 60° C. before the neutralizing step,
- wherein the bioactive molecule is natamycin,
- wherein the base is an organic base chosen from the group consisting of choline hydroxide, choline chloride, choline bitartrate, choline monohydrogen-tartrate, choline-dihydrogen-citrate, branched polyethyleneimine, tetramethylammonium hydroxide, tetraethylammonium hydroxide, and tetrabutylammonium hydroxide,
- wherein the composition is an aqueous solution,
- wherein the natamycin is present in a range between 3 and 18 wt. %, relative to the total weight of the neutralized composition, and
- wherein the acid is selected from the group consisting of formic acid, acetic acid, propionic acid, sorbic acid, benzoic acid, malic acid, butanoic acid, hexanoic acid, hydroxybutanedioic acid, fumaric acid, tartaric acid, ascorbic acid, octanoic acid and nicotinic acid,
- wherein the amount of organic base ranges from 4-30 wt. % relative to the total weight of the composition,
- wherein the PEG is present in an amount from 30-90 wt. % based on the total weight of the composition,
- wherein the natamycin is stable in the aqueous solution as evidenced by lack of crystallization after 24 hours.
A process for preparing a composition comprising a natamycin comprising the steps of:
- a) dissolving the natamycin with an aqueous base at OW 1 to obtain a solution A including an ionic complex of the natamycin and the base,
- wherein the base is one or more of potassium hydroxide and sodium hydroxide,
- b) heating PEG to a temperature between 30° C. and 60° C.;
- c) adding the heated PEG to solution A and mixing therewith to form Solution A′, and;
- d) neutralizing the solution A′ with an acid to obtain the composition comprising the natamycin having a pH between 6 and 9,
- wherein the acid is one or more of formic acid, acetic acid, propionic acid, sorbic acid, benzoic acid, lactic acid, malic acid, butanoic acid, hexanoic acid, hydroxybutanedioic acid, citric acid, fumaric acid, tartaric acid, ascorbic acid, octanoic acid and nicotinic acid;
- wherein the natamycin is present in an amount from 3-18 wt. % based on the total weight of the composition,
- wherein the base is present in an amount from 4-30 wt. % based on the total weight of the composition,
- wherein the PEG is present in an amount from 30-90 wt. % based on the total weight of the composition, wherein the composition is an aqueous solution, and wherein the natamycin is stable in the aqueous solution as evidenced by lack of crystallization after 24 hours.
Dependencies are inferred from the claim text.