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US 11,835,532 B2Granted
Green concentrated reagent for hemotology systems
AssigneeBeckman Coulter, Inc.
InventorsIgnacio Figueroa, Jing Li, Nery Ortiz, Wayne Goldson
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Abstract
Environmentally-friendly, aqueous concentrated reagent compositions are provided for dilution and use in suitable hematology analyzers for analyzing blood cells including for enumeration and sizing of blood cells, determination of hemoglobin parameters and differentiation of leukocyte subpopulations in a single blood cell sample.
Claims20
A concentrated reagent composition comprising:
- a metal sulfate;
- a phosphate-based buffer;
- a hemoglobin ligand;
- and
- an antimicrobial agent;
- wherein the concentrated reagent composition is diluted with water by a dilution factor of between 10 and 20 to provide a working concentration reagent composition, wherein the working concentration reagent composition is used in a hematology analyzer for analyzing cells.
3 more elements
A method for analyzing a biological sample comprising:
- providing a concentrated reagent composition having a metal sulfate, a phosphate-based buffer, a hemoglobin ligand, and an antimicrobial agent;
- diluting the concentrated reagent composition with water by a dilution factor from between 10-fold to 20-fold to form a working concentration reagent composition;
- utilizing a hematology analyzer to mix a biological sample with the working concentration reagent composition to form a diluted biological sample;
- and,
- analyzing the diluted biological sample to determine at least one physical parameter of the sample.
2 more elements
Dependencies are inferred from the claim text.