Process for purification of a sialic acid from a fermentation broth
AssigneeChr. Hansen HMO GmbH
InventorsStefan Jennewein, Markus Helfrich
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Abstract
The present invention describes an efficient way to isolate a sialic acid from a fermentation broth. The sialic acid contained in the fermentation broth is produced by bacterial fermentation. The inventive process comprises a step of removing biomass from the fermentation broth, a step of subjecting the resulting solution to at least one of a cationic ion exchanger treatment and an anionic ion exchanger treatment and a step of removing salts after the ion exchanger treatment. The process can provide the sialic acid in spray-dried form as well as in form of sialic acid crystals.
Claims34
A process for purification of a sialic acid from a fermentation broth, comprising:
- removing biomass from a fermentation broth comprising a sialic acid, wherein a clarified solution is provided,
- providing a purified solution by subjecting the clarified solution to
- a cationic ion exchanger treatment with a cationic ion exchanger material, wherein the cationic ion exchanger treatment is performed under conditions in which the sialic acid passes the cationic ion exchanger material and is present in the flowthrough;
- and
- an anionic ion exchanger treatment with an anionic ion exchange material, wherein the anionic ion exchanger treatment is performed under conditions in which the sialic acid passes the anionic ion exchanger material and is present in the flowthrough;
- and
- removing salt from the purified solution by electrodialysis.
Dependencies are inferred from the claim text.