Method for producing hypophysis precursor tissue
AssigneesRiken, Sumitomo Chemical Company, Limited
InventorsYoshiki Sasai, Hidetaka Suga
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Abstract
The present invention provides a method of obtaining aggregates containing a rostral hypothalamus tissue and a rostral head ectodermal tissue, a hypophysis precursor tissue and a hypophysis hormone producing cell, by using a serum-free medium (preferably substantially free of growth factor and insulins), forming homogeneous aggregates of stem cells from pluripotent stem cells such as ES cell and the like, which are plated at a high cell concentration, and subjecting the formed aggregates to floating-culture.
Claims12
An aggregate comprising both a hypothalamus tissue and a non-neural head ectoderm and having been induced in vitro to differentiate from a pluripotent stem cell, wherein the non-neural head ectoderm is present on a surface layer of the aggregate and the hypothalamus tissue is present inside thereof.
Dependencies are inferred from the claim text.