Methods and compositions for identifying epitopes
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsStephen Elledge, Tomasz Kula, Mohammad Haj Dezfulian
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Abstract
Described herein, in one aspect, are antigen presenting cells (APCs) comprising an exogenous nucleic acid encoding one or more candidate antigens, wherein the one or more candidate antigens are expressed and presented with MHC class I or MC class II molecules; a molecular reporter of Granzyme B (GzB) activity; and c) an exogenous inhibitor of caspase-activated deoxyribonuclease (CAD)-mediated DNA degradation, a CAD knockout, or a caspase knockout (e.g., caspase 3 knockout). Described herein, in another aspect, is a system for detection of recognized antigen presentation by an antigen presenting cell to a cytotoxic lymphocyte or NK cell.
Claims22
An antigen presenting cell (APC) comprising:
- a) an exogenous nucleic acid encoding one or more candidate antigens, wherein the one or more candidate antigens are expressed and presented with WIC class I or WIC class II molecules;
- b) a molecular reporter of Granzyme B (GzB) activity;
- and
- c) an exogenous nucleic acid encoding inhibitor of caspase-activated deoxyribonuclease (ICAD) in expressible form, an exogenous inhibitory nucleic acid targeting caspase-activated deoxyribonuclease (CAD), a CAD knockout, or a caspase knockout.
Dependencies are inferred from the claim text.