In vitro immune synapse system and method of in vitro evaluating immune response using the same

A method of in vitro evaluating immune response, comprising:

• providing an in vitro immune synapse system, wherein the in vitro immune synapse system comprises:
• an antigen-presenting cell, wherein the antigen-presenting cell is an alveolar macrophage;
• at least one of a regulatory T cell, a killer cell and a helper T cell, wherein the regulatory T cell has at least a CD4+/CD25+ cell marker, the killer cell has at least a CD4−/CD25+ cell marker, the helper T cell has at least a CD4+/CD25− cell marker;
• and
• a control cell, wherein the control cell has at least a CD4−/CD25− cell marker, and
• wherein the antigen-presenting cell, the regulatory T cell, the killer cell, the helper T cell and the control cell are primary cell derived from a same individual, and a number of the antigen-presenting cell is 2 to 10 times of a total number of the regulatory T cell, the killer cell and the helper T cell;
• co-culturing a test sample in the immune synapse system for 24 hours;
• and
• detecting the immune synapse system for an immunization-related change, wherein the immunization-related change comprises a level of toll-like receptor (TLR) gene expression, and
• wherein when any one of the regulatory T cell, the killer cell, the helper T cell has its immunization-related change significantly differ from the immunization-related change of the control cell, the test sample is determined to be immunogenic or immunostimulatory in a porcine respiratory mucosa immune system.