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US 11,833,503 B2Granted
Methods and compositions for surface functionalization of optical semiconductor-integrated biochips
AssigneeInSilixa, Inc.
InventorsAndrea Cuppoletti, Arjang Hassibi, Lei Pei, Yang Liu, Kshama Jirage, Arun Manickam
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Abstract
The present disclosure provides methods and compositions for surface functionalization of solid substrates. The compositions include functionalized silanes and nucleic acid constructs which may react to immobilize the nucleic acid constructs on the surface on the solid substrate. The disclosure also provides methods for immobilization of silanes and nucleic acid constructs on the surface of the substrate.
Claims14
A method for immobilizing a nucleic acid construct to a surface of a substrate, the method comprising:
- immobilizing a silane to the surface of the substrate, wherein the silane comprises a first chemical group;
- and
- contacting the silane with the nucleic acid construct comprising a second chemical group, wherein the contacting is performed for less than 10 minutes;
- wherein the substrate comprises an integrated optical sensor circuit comprising a complementary metal-oxide-semiconductor (CMOS) integrated circuit (IC), wherein (1) the first chemical group comprises a first alkyne and the second chemical group comprise a first azide;
- or (2) the first chemical group comprises a second azide and the second chemical group comprises a second alkyne, wherein the first and second alkyne is a dibenzocyclooctyne (DBCO), or a derivative thereof, wherein the first chemical group reacts with the second chemical group via a strain-promoted alkyne-azide cycloaddition (SPAAC), thereby immobilizing the nucleic acid construct on the surface of the substrate.
2 more elements
Dependencies are inferred from the claim text.