System and method for emulsion breaking
AssigneeLife Technologies Corporation
InventorsBrian Reed, James A. Ball
Life of this patent
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Abstract
A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
Claims15
A method of processing a bead support comprising:
- breaking an emulsion including a sample of bead supports in a dispersed aqueous phase by applying the emulsion to a centrifuge tube while centrifuging;
- the bead supports preferentially partitioning into a surfactant solution covered by a hydrophobic liquid;
- washing the bead supports recovered from the surfactant solution;
- and
- applying the washed bead supports to an array of wells, wherein the array of wells forms a part of a genetic sequencing system for performing genetic testing on the bead supports.
Dependencies are inferred from the claim text.