Norovirus S particle based vaccines and methods of making and using same
AssigneeChildren's Hospital Medical Center
InventorsMing Tan, Xi Jason Jiang
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Abstract
Disclosed herein are vaccine compositions, in particular, polyvalent icosahedral compositions for antigen presentation. The disclosed compositions may contain an S particle made up of recombinant fusion proteins. The recombinant fusion proteins may include a norovirus (NoV) S domain protein, a linker protein domain operatively connected to the norovirus S domain protein, and an antigen protein domain operatively connected to said linker.
Claims18
A polyvalent icosahedral composition for antigen presentation comprising an S particle, wherein said S particle comprises a recombinant fusion protein comprising
- a) a norovirus (NoV) S domain protein comprising a mutation at position 69, and a mutation selected from 57C/140C, 57C/58C/136C, and 57C/58C/136C/140C;
- b) a linker protein domain operatively connected to said norovirus S domain protein;
- and
- c) an antigen protein domain operatively connected to said linker;
- wherein said mutations are with reference to SEQ ID NO:
- 25;
- wherein said mutation at position 69 renders a cleavage site between R69 and N70 resistant to trypsin cleavage;
- and
- wherein said norovirus is a human calicivirus.
A recombinant fusion protein comprising
- a) a norovirus (NoV) S domain protein comprising a mutation at position 69, and a mutation selected from 57C/140C, 57C/58C/136C, and 57C/58C/136C/140C;
- b) a linker protein domain operatively connected to said norovirus S domain protein;
- and
- c) an antigen protein domain operatively connected to said linker;
- wherein said mutation at position 69 renders a cleavage site between R69 and N70 resistant to trypsin cleavage;
- wherein said mutations are with reference to SEQ ID NO:
- 25;
- and
- wherein said norovirus is a human calicivirus.
Dependencies are inferred from the claim text.