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US 11,833,187 B2Granted
Materials and methods for extracellular vessicle-associated diseases
AssigneeThe Brigham and Women's Hospital, Inc.
InventorsShinsuke Ito, Elena Aikawa, Masanori Aikawa
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Abstract
Sortilin is a sorting receptor that directs target proteins to the secretary or endocytic compartments of cells that is found in both extracellular vesicles and cells. Provided herein are methods and compositions for decreasing or inhibiting trafficking of sortilin to an extracellular vesicle, for example by inhibiting the formation of intermolecular sortilin dimers.
Claims9
A method for identifying a test agent that modulates dimerization of sortilin, the method comprising:
- (i) contacting a cell with a test agent, wherein the cell expresses a first sortilin polypeptide comprising a first label, and a second sortilin polypeptide comprising a second label, wherein the first and second sortilin polypeptide comprises independently the amino acid sequence of SEQ ID NO:
- 1;
- and
- (ii) detecting a contact level between the first and second sortilin polypeptide expressed in the cell,
- wherein a change in contact level relative to a control or reference level indicates the agent modulates dimerization of sortilin.
2 more elements
Dependencies are inferred from the claim text.