Nano-ligand for promoting cell adhesion and differentiation of stem cells and method of promoting cell adhesion and differentiation of stem cells by using the same

A method of promoting cell adhesion and differentiation of stem cells, the method comprising:

• manufacturing a nano-ligand presenting substrate by putting a substrate, of which a surface is activated, in a solution including a nano-ligand for promoting adhesion and differentiation of stem cells, the nano-ligand comprising a core including magnetic nano-particles, a coating layer provided to surround the core and including an integrin-binding ligand peptide, and a linker provided between the core and the coating layer, wherein the integrin-binding ligand peptide is negatively charged;
• and
• controlling adhesion and differentiation of stem cells by treating the nano-ligand presenting substrate with stem cells and then applying an external magnetic field,
• wherein the manufacturing of the nano-ligand presenting substrate includes:
• soaking the surface of the substrate in an acid solution;
• activating the surface of the substrate by putting the soaking-completed substrate in an amino-silane solution;
• and
• treating the activated substrate by using ultrasonic waves at a room temperature.